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KMID : 0903520090520040336
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
2009 Volume.52 No. 4 p.336 ~ p.341
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Isolation and Identification of Lipids from the Silkworm (Bombyx mori) Droppings
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Sohn Bong-Hee
Park Ji-Hae
Lee Dae-Young
Cho Jin-Gyeong
Kim Yong-Soon
Jung In-Sik
Kang Pil-Don
Baek Nam-In
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Abstract
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Silkworm (Bombyx mori) droppings were extracted with 80% aqueous MeOH, and the concentrated extract was partitioned successively with EtOAc, n-BuOH, and H2O. Three lipids were isolated from the EtOAc fraction by repeated silica gel and ODS column chromatography. Physico-chemical data including NMR, MS, and IR indicated that the isolated lipids were β-sitosterol (1), corchorifatty acid E methyl ester (2), and (8E)-10-hydroxydec-8-enoic acid methyl ester (3). Compounds 2 and 3 have, prior to this study, not previously been documented in nature.
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KEYWORD
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Bombyx mori, corchorifatty acid E methyl ester, (8E)-10-hydroxydec-8-enoic acid methyl ester, hydroxy-unsaturated fatty acid, silkworm droppings, β-sitoster
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